Hello! This is Seongchelin Guide😊
Selected from my region Stem cells (ADSC),
If you freeze it properly, it can be useful someday!
Have you ever heard a story like this?
However where entrust it to how must be kept
Is it really safe?
Today, instead of difficult medical terms
Refrigerator, Freezer, Time Capsule With the same easy example
Stem cell storage methods and
Cell health check (QC) I will summarize the process for you.
What is banking? The concept of depositing cells in a vault.
What is banking?
Banking (bank + ing)
Just like saving money in a bank
Cells, blood, embryos My back
It is stored in a dedicated facility!
There are characteristics depending on the storage temperature.
| Temperature | alias | 보관 기간 | Exclamation mark |
| 4 ° C | refrigerator | A few days | When writing again today and tomorrow |
| -80 ° C | laboratory freezer | 6 months – 2 years | Medium-term storage |
| -196 ° C | 액체질소탱크 | 5 – 10 years | “Cell Time Capsule!” |
Why do stem cell banking?
Stem cells are the all-purpose toolbox inside my body.
Adipose-derived stem cells (ADSCs) fill damaged tissue or
It can transform into new fat, bone, blood vessels, etc.
It can be harvested relatively simply, like liposuction, and
It also has a low immune rejection reaction. Self-recycling It's a feeling.
The moment when it would be good to collect stem cells and use them in the future is
There are times like this.
| situation | example |
| Volume replenishment | When the shape of the breasts changes due to childbirth or weight changes |
| Wound and Scar Care | Filling in surgical scars or depressions after trauma |
| Anti-aging | Fat grafting for maintaining facial volume and elasticity |
As you get older, both the number and strength of your stem cells decrease.
Cells set aside when one is young and healthy Cell ageSince it stops right there, you can use it fresh even 10 years later.
So, we are banking stem cells starting now.
–196 °C banking 10 The reason it is a 'time capsule'
1. Stopping chemical reaction
-196 °C is the temperature of liquid nitrogen.
If you make it this cold The clock inside the cellStop.
2. At –196 °C, intracellular metabolic and enzyme activity is almost 0 :
→ Aging is temporarily paused.
3. Ice crystal inhibition:
Protectant DMSO 5–10%
Block the 'ice arrow' cell membrane damageMinimize it.
4. Contamination Prevention :
This temperature line even freezes bacteria and mold into ice cubes!
→ Concerns about external contamination ↓
5. Proven with Data :
Research results of the paper,
3 years · 5 years · 1 at –196 °C
I tried thawing it after storing it for 0 years.
More than 78 out of 100 cellsHe was alive and well,
It also maintained the function of transforming into new adipose tissue.
Simply put, even if you sleep for 10 years and wake up
8 out of 10 cellsIt was still alive just as it was!
Cell health check (QC)
― Cell Health Checkup!
Essential for withdrawing money with confidence in banking!
| step | Test details | Easy explanation |
| ① Exterior Check | Check container, label, and discoloration | 'Cell Container Outpatient Inspection'—Packaging OK? |
| ② Measurement of survival rate | Microscope count after staining | Calculate the ratio of living cells (green) to dead cells (red). |
| ③ Sterility test | Bacteria and fungi culture | If it gets murky, you're out! You must be clean to pass. |
| ④ Toxin test | Endotoxins and fine pollutants | Confirmed “No Poison” with Ultra-Fast Strip Kit |
| ⑤ Function verification | Differentiation and proliferation ability spot test | Is 'transformation and proliferation' possible after a short culture period? |
QC is a cell plate health checkup!
Must pass: “This cell is exactly the same as it was 10 years ago”
You could say that.
Cells are like people Health screeningsYou must receive
You can use it with peace of mind.
Banking Procedure A → Z
In that case, let's look at the cell banking procedure.
1. Collection : In the abdomen or thighs
Aspirate a small amount of fat, 100-200 mL
2. Separation : With a mechanical extractor
Separation of subcutaneous stem cell families (SVF) from adipose tissue
3. Mixing of protective agent : Mix about 10% DMSO
Ice crystal prevention
4. Stage cooling : Slowly freezing by decreasing by -1 °C
You have to freeze it slowly The cell isn't surprised.Cell dehydration prevention)
5. Liquid Nitrogen Tank Storage :
Stored in a -196 °C safe, real-time alarms for temperature and nitrogen levels
6. Request thawing : Customer requests thawing reservations if needed (usually 1 week in advance)
7. Fast Thawing · QC : 2 minutes in 37 °C water,
Next, proceed with QC Step 4.
You have to melt it quickly Blocks ice crystal regeneration.
And immediately after thawing, DMSO,
In other words, it thoroughly washes away the cytoprotective agent. → Remove to less than 0.1% before administration to the human body.
8. Use : Injection into the target site or filling tissue gaps
“Will It Fit Me?” Simple Checklist
1 Long-term planning
(Volume restoration after pregnancy and childbirth, etc.)
→ YES, Banking Recommendation
2. Not used within 6 months
→ –80 °C is enough,
However, -196 °C is safe when it lasts longer than a year.
3. Quality concerns
Banking companies and hospitals
QC ReportMake sure to check if it is issued!
Even 10 years from now “Safely living cells”,
The fact that it is technically fully possible!
The key is –196 °C ultra-low temperatureand
Cell health check (QC)
It means taking care of both things.
Banking, which seemed so difficult,
Are you feeling a little more familiar with it now? 😊
If you have any questions, please feel free to leave a comment.
Next time too, easy and fun
I'll be back with a story about plastic surgery.
Thank you for reading!
references
Kostecka, A., Kalamon, N., Skoniecka, A., Koczkowska, M., Skowron, P.M., Piotrowski, A., & Pikuła, M. (2024). Adipose-derived mesenchymal stromal cells in clinical trials: Insights from single-cell studies. Life Sciences, 351, 122761. https://doi.org/10.1016/j.lfs.2024.122761
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